2-in-1 SARS-CoV-2 Protein Microarray

Profile the SARS-CoV-2 Antibody Response

Understanding the SARS-CoV-2 humoral immune response of the COVID-19 patients and vaccinated people is valuable research that supports efforts to prevent, diagnose and treat COVID-19. 

Analyse your patient serum samples for SARS-CoV-2 specific antibodies with our 2-in-1 SARS-CoV-2 Microarray. The microarray combines a full-length protein array to determine which viral proteins are immunodominant, with a peptide array to examine epitope-level binding to the spike protein.

 

2-in-1 SARS-CoV-2 Protein Microarray – Sample Layout

Sample layout of 2-in-1 SARS-CoV-2 Protein Microarray – 14 sets per printed slide

  • Most proteins are His-tagged and are visualized by an anti-6xHis antibody followed by a fluorescent-labelled secondary antibody (Red)
  • Peptides are chemically conjugated to BSA and visualized by an anti-BSA antibody followed by a fluorescent labelled secondary antibody (Green)
  • NSP2, NSP5, NSP14, NSP15, NSP16, S1-4 and E protein are tagged with eGFP; ACE2 has a human Fc tag

 

From the developer

“The 2-in-1 SARS-CoV-2 protein microarray is a tool-of-choice for systematical profiling of SARS-CoV-2 humoral responses in a fast and high-throughput way,” says Sheng-ce Tao, who first developed and published this assay with his team at Shanghai Jiao Tong University, China. “We and others have successfully applied this microarray for analyzing thousands of serum samples, and constructed a comprehensive map of SARS-CoV-2 specific antibody responses. The results from these studies are guiding us for in-depth understanding and clinical applications.”

Tao Lab

The Tao Lab


More resources

Download detailed product information and user protocol

Watch webinar: Protein Microarrays for Systematical Deciphering of SARS-CoV-2 Specific Humoral Immune Responses

Read article: Conversation with a COVID-19 researcher

 

References

Jiang et al. 2020. SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses, Nature Communications

Li et al. 2020. Linear epitopes of SARS-CoV-2 spike protein elicit neutralizing antibodies in COVID-19 patients, Cellular and Molecular Immunology

Li et al. 2020. Systematic evaluation of IgG responses to SARS-CoV-2 spike protein-derived peptides for monitoring COVID-19 patients, Cellular and Molecular Immunology

Lei et al. 2020. Antibody dynamics to SARS‐CoV‐2 in asymptomatic COVID‐19 infections, European Journal of Allergy and Clinical Immunology

Tao et al. 2021. Antibody landscape against SARS-CoV-2 reveals significant differences between non-structural/accessory and structural proteins, Cell Reports

Li et al. 2021. Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients, Cell Reports