Arrayjet inkjet technology vs. pin-spotting

   Contact printer  Arrayjet non-contact inkjet bio-printer
Pins or piezo?  Frequent replacement of expensive pins that wear out, break easily or become blunt. Tip systems also require calibration and complicated routines to input specific parameters.  As a result, set-up costs are high. Non-contact inkjet piezo-electric technology with a 126 nozzle print head that ejects samples 'on-the'-fly', without causing denaturation. Less frequent maintenance is possible as parts have a long shelf life and do not require regular replacement.
 Flexibility Fixed pin placement limits flexibility of array layout, spacing and design. Compact print head offers complete flexibility and range of movement, less than 1mm above the substrate.  Allowing complete control of spacing, alignment and array layout.  Accurate spot-on-spot deposition enables printed volumes of 100 pL to 10 nL in a single run.
 Speed and efficiency Mid-range printing speed. Limited pin flexibility drastically increases print time of higher density arrays. Fastest printing technology in the market. Ability to print up 640 features per second.
 Throughput Low yield and low throughput. Not possible to print large batches of slides whilst maintaining speed. Multiple sample loading from 6 or 48 source plates loaded simultaneously. Capacity to print up to 1000 chips or slides, or 200 microplates in a single run.
 Sample type Sample restrictions with some systems.  Higher viscosity samples tend to block pins. Potential to print a full range of samples including proteins and peptides, nucleic acids, small molecules, antibodies, lysates and carbohydrates.
 Sample conservation Larger dead volumes contribute to higher sample waste. Sample-aspirating Jetspyder™ reduces dead volume and required aspiration volume.  Valuable samples are conserved and sample waste is minimal.
 Sample evaporation Slower print runs can result in sample evaporation and concentration changes. Evaporation and concentration changes are preventable with use of Arrayjet Jetguard™ seals. Arrayjet environment control unit regulates extreme temperature and humidity changes for all environments.
 Surface chemistries Not compatible with increasingly popular coatings such as hydrogel. Compatible with slides, MEMs and microfluidic chips, microneedles, 384-well microplates. Non-contact technology does not damage the substrate surface coating, nor does it affect sample binding.
 Missing Spots Potential for missing features caused by drying of samples during long print runs, or from samples not sticking to the pin itself.  Samples can run out before all the spots have been deposited, or may not stick to the pins at all.  If pins are not cleaned effectively, samples may carry over and disrupt quality of data. Few or no missing spots as print head is loaded fully with liquid, capable of dispensing a full volume of drops.  User-defined cleaning routines purge and clean the system between each aspiration to eliminate potential for contamination.
 "Doughnut" effects Contact with substrate creates a ring-shaped spot with poor sample distribution.   Non-contact printing results in equal sample distribution, allowing for more accurate data analysis.
 Optical QC camera No inbuilt real-time quality control optical system. Iris™ optical QC system detects defects, addresses real time print issues and creates a customisable QC data report based on user-defined threshold criteria. Command Centre Pro-License can reprint any missing features.