Arrayjet inkjet technology vs pin spotting

   Contact Printer  Arrayjet Non-Contact Inkjet Bioprinter
Pins or Piezo  Contact printer requires frequent replacement of pins that can be expensive and wear out, break easily or go blunt. Tip systems also require calibration. The contact printer uses complicated set up routines to input all the parameters and therefore, higher set up costs. Arrayjet uses inkjet, non-contact ‘on the fly’ piezo electric technology with 126 nozzled print head that ejects the samples without any wear and tear. Lower maintenance due to long shelf life and no regular part replacement involved.
 Flexibility Lower flexibility due to the fixed pin placement. Limited control spacing, layout and design restrictions. Complete flexibility due to compact print head and ability to move with total movement of range across the substrate (40 μm diameter). Ideal choice for multiplex assays. Complete control of sample spacing, alignment, layout and volumes dispensed in 100pL droplets. Spot-on-spot up to 10nL in one batch run.
 Speed and Efficiency Middle of the market print speed; reasonable speed. Limited pin flexibility drastically increases print time when performing higher density custom runs. Fastest printing technology in the market. Ability to print up 540 features per second.
 Throughput Lower yield and throughput. Cannot print larger batches of slides for high-throughput manufacturing while maintaining the speed. The system can support multiple sample loading with 6 source plates or 48 source plates loaded at a time. The capacity to print per batch is up to 1000 chips or slides and 200 microplates.
 Sample type Some contact spotters have sample type restrictions. Ability to print a full range of samples such as proteins, DNA, RNA, peptides, small molecules, antibodies, proteins, lysates, carbohydrates.
 Sample Wastage Requires larger dead volumes and have higher sample wastage. The sample aspirator ‘Jetspyder’ reduces the dead volume required and also the aspiration volume required, thereby preserving valuable samples.
 Sample Evaporation Slower printruns can lead to sample evaporation and concentration changes. Evaporation and concentration changes can be prevented using Arrayjet Jetguard™ seals. Arrayjet environment control unit regulates extreme temperature and humidity changes for all environments.
 Surface chemistries Use coatings such as hydrogels, which are increasingly popular for their increased binding capacity, a contact pin spotter would not be suitable. Ability to print on to slides, MEMs chips, microfluidic chips, microneedles, 384-well microplates etc. There is no damage to the coating on slide surfaces or the sample itself.
 Missing Spots Missing features observed. This could be due to samples drying out during long print runs from evaporation or from samples not sticking to the pin itself or running out of sample before all the spots have been deposited. Samples may stick to the pins and not clean off, causing 'carry over' between samples and disrupting data quality. Few or no missing spots observed with inkjet printing as the printhead is loaded fully with liquid and will dispense the full volume of drops. There are defined cleaning routines that purge and clean the system between each group of samples that are loaded to eliminate any contamination.
 Donut effects Donut effect seen. A ring-shaped spot can be seen because the contact has created a poorly shaped spot. This often looks like a circle with a different coloured/intensity ring around it. No donut effect observed with Arrayjet inkjet printhead.
 Optical QC camera No inbuilt real-time quality control optical system. Iris optical QC™ automatically detects defects, addresses real time print issues and customises QC data report and threshold criteria. Command Centre Pro-License can reprint any missing features.