In this section
- Antibody specificity
- Antigen discovery
- Biomarker discovery and serum profiling
- Compound library screening
- Epitope mapping
- Gene expression profiling
- Human glycomics
- Hybridoma screening
- Label-free SPRi
- Microbiome analysis
- Plant glycomics
- Point of care diagnostics
- Reverse phase protein arrays (RPPA)
- Single cell analysis
Antibody specificity testing
Antibodies are vital research tools, but unless properly validated they can yield inconsistent data, wasting time, money and valuable samples.
Arrayjet customers have utilised protein microarray to independently validate thousands of antibodies against cross-reactivity and batch-to-batch variation.
High-density antigen microarrays for the assessment of antibody selectivity and off-target binding
R. Sjoberg, E. Andersson, C. Hellstrom, C. Mattsson, J. M. Schwenk, P. Nilsson, and B. Ayoglu
Methods in Molecular Biology
"Our Arrayjet Marathon has been instrumental in achieving the throughput and reliability that have been needed for the production of antigen arrays for validation of 47,000 antibodies within the Human Protein Atlas. It has also shown to be highly suitable for the production of our large-scale arrays that comprise over 20,000 features per array in both antigen and reverse phase serum arrays. We have arrayed more than 12,000 slides with the Arrayjet Marathon over the last five years and it has become a very important part of our research group and the SciLifeLab National Affinity Proteomics protein array facility."
Peter Nilsson, Principal Investigator, The Human Protein Atlas, Stockholm