Plant glycomics

Plant glycomics

Glycan arrays offer significant advantages for glycomics research - mainly, in that a broad range of multivalent glycans can be immobilised on a solid surface and interrogated simultaneously.  High density glycan arrays can be printed using Arrayjet microarray spotters, enabling the analysis of up to 75,000 binding events on a single microarray slide.

Researchers at the University of Copenhagen use a Marathon Argus non-contact microarray spotter to print their glycan arrays.

 

Application note

Carbohydrate Microarray Application Note.pdf

Selected publications

Double blind microarray-based polysaccharide profiling enables parallel identification of uncharacterized polysaccharides and carbohydrate-binding proteins with unknown specificities
A. A. Salmeán, A. Guillouzo, D. Duffieux, M. Jam, M. Matard-Mann, R. Larocque, H. L. Pedersen, G. Michel, M. Czjzek, W. G. T. Willats, and C. Hervé
Nature Scientific Reports

High throughput screening of starch structures using carbohydrate microarrays
Vanja Tanackovic, Maja Gro Rydahl, Henriette Lodberg Pedersen, Mohammed Saddik Motawia, Shahnoor Sultana Shaik, Maria Dalgaard Mikkelsen, Susanne Langgaard Krunic, Jonatan Ulrik Fangel, William George Tycho Willats and Andreas Blennow
Nature Scientific Reports

Microarray-based polysaccharide profiling enables parallel identification of uncharacterized polysaccharides and carbohydrate-binding proteins with unknown specificities
Armando A. Salmeán, Alexia Guillouzo, Delphine Duffieux, Murielle Jam, Maria Matard-Mann, Robert Larocque, Henriette L. Pedersen, Gurvan Michel, Mirjam Czjzek, William G. T. Willats and Cécile Hervé
Nature Scientific Reports


Testimonials

"The production of microarrays of complex and diverse carbohydrates presents particular unique challenges. The Arrayjet microarray spotter works very well for this purpose and in our experience, is superior to pin based printers. We have found that the Arrayjet printer is very fast, accurate and most important, able to handle polysaccharides and oligosaccharides with widely different chemical and physical properties. What is also impressive is that we are able to print extracted material directly without clean up, despite the fact that some extraction solvents are very harsh, for example 4 M sodium hydroxide.''

Prof. William Willats, University of Newcastle